Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers

Table 3 Effects of nitrate on relative abundance of methanogenic genera

Item	Treatments			SEM	P-value	Contrast
Item	0NR	1NR	2NR	SEM	P-value	P _L	P _Q
vadinCA11	56.15	54.15	58.34	2.62	0.51	0.55	0.32
Methanobrevibacter	36.64	35.43	32.89	2.20	0.49	0.25	0.81
Methanosphaera	0.8100^b	0.8550^b	1.3575^a	0.0977	0.0057	0.0033	0.0881
Methanimicrococcus	0.1000^b	0.1017^b	0.2300^a	0.0333	0.0199	0.0113	0.0979
Methanoplanus	0.0033^a	0.0012^b	0.0009^b	0.0005	0.0028	0.0013	0.1508
Methanosarcina	0.0029	0.0045	0.0028	0.0007	0.2141	0.9550	0.0855
Methanobacterium	0.0019	0.0014	0.0013	0.0004	0.4066	0.2816	0.4590
Methanoculleus	0.0003^b	0.0010^a	0.0003^b	0.0002	0.0417	1.0000	0.0140

0NR control, 1NR 1% nitrate, 2NR 2% nitrate, P_L is liner tendency; P_Q is quadratic tendency

ISSN: 1471-2180