Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers

Table 2 Effects of nitrate on relative abundance of methanogen order of rumen

Items	Treatments			SEM	P-value	Contrast
Items	0NR	1NR	2NR	SEM	P-value	P _L	P _Q
E2	56.15	54.15	58.34	2.62	0.51	0.55	0.32
Methanobacteriales	38.00	35.68	34.62	2.12	0.42	0.20	0.80
Methanomicrobiales	0.47^a	0.25^b	0.27^b	0.06	< 0.05	0.05	0.13
Methanosarcinales	0.06^b	0.12^b	0.24^a	0.03	0.01	< 0.01	0.40

0NR control, 1NR 1% nitrate, 2NR 2% nitrate, P_L is liner tendency, P_Q is quadratic tendency

ISSN: 1471-2180