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Table 7 Mutations in DnaA-boxes affect copy number of the MiniR1–1 plasmid

From: Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid

Strain

Relative copy number of plasmid

MG1655/pACYC177

11 (±3.0)

MG1655/MiniR1–1

1 (±2.7)

MG1655/pbox1CA-TG

6 (±1.3)

MG1655/pbox1TT-GG

6 (±1.7)

MG1655/pbox3TA-GG

2 (±0.7)

MG1655/pORF2M-V

Not determined

MG1655/pbox8TT-GG

5 (±1.8)

MG1655/pORF111L-W

1 (±0.6)

  1. Slowly growing wild-type MG1655 cells carrying individaul plasmid listed were collected for Q-PCR. Copy number of the plamids relative to the chromosomal terC site was determined by Q-PCR as described in Methods. The values are the average of three experiments, the standard errors are as shown in brackets
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BMC Microbiology

ISSN: 1471-2180

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