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Table 5 The presence of MiniR1–1 results in an increase in cell size

From: Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid

Strain

Cell size (μm)

Doubling time (min)

MG1655

2.2(±0.1)

196(±1.5)

MG1655/pACYC177

2.2(±0.2)

185(±1.5)

MG1655/MiniR1–1

2.7(±0.1)

195(±1.5)

MG1655/pbox1CA-TG

3.2(±0.4)

200(±1.1)

MG1655/pbox1TT-GG

3.3(±0.2)

229(±1.0)

MG1655/pbox3TA-GG

2.5(±0.3)

198(±1.5)

MG1655/pORF2M-V

No growth

MG1655/pbox8TT-GG

2.0(±0.1)

198(±1.1)

MG1655/pORF111L-W

2.8(±0.1)

188(±1.5)

  1. Wild-type MG1655 cells carrying the plasmids listed above were grown at 30 °C in ABT medium, harvested at OD450 = 0.15, and analyzed by fluorescence microscopy as described in Methods. The values are the average of three experiments, the standard errors are as shown in brackets