Table 2 Primers used
From: Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid
Name of primer | Sequence |
|---|---|
DnaA-box1CA-TG | F5’-CGGGGAATTTTGCTTATCTGCATTTAACTGG R5’-CAGATAAGCAAAATTCCCCGTCGCTGAG |
DnaA-box1TT-GG | F5’-GACGGGGAATTTTGCGGATCTGCATTTAAC R5’-CAGATCCGCAAAATTCCCCGTCGCTGAG |
DnaA-box3TA-GG | F5’- CGATATCTGAATTGTTAGGCATGTG R5’- CCTAACAATTCAGATATCGTTACCAGG |
ORF2M-V | F5’- CCGGGGGATTGTGCTGAACGTCTTC R5’- CAATCCCCCGGAACAGCGTTTCCTC |
DnaA-box8TT-GG | F5’- GTTGCAAGAAAACGTCGGACACAC R5’- CCGACGTTTTCTTGCAACAGCGGC |
ORF111L-W | F5’-GCTTCACCTCCCGTTTGGATTTC R5’-CAAACGGGAGGTGAAGCCCACC |
bla | R5’- AGCCATACCAAACGACGAGC |
R5’- TCAGCAATAAACCAGCCAGC | |
terC | R5’-TCCTCGCTGTTTGTCATCTT |
R5’-GGTCTTGCTCGAATCCCTT | |
dnaA | F5’-GCTCTAGAGGTGTCACTTTCGC |
R5’-GGGGTACCCGCGATGACAATGTTCTG | |
repA | F5’-AACTGCAGGGTGACTGATCTTCAACAAAC |
R5’-CGGGATCCTCGGGGGAAGCTGTGGCCAGC |