Fig. 1
From: Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid
The presence of MiniR1–1 plasmid leads to an accumulation of the D-period cells. a Wild-type MG1655 cells without or with pACYC177 or MiniR1–1 plasmids were grown in ABT medium at 30 °C and fixed in 70% ethanol in the log phase. The cell cycle parameters were measured by flow cytometry. b Exponentially growing cells described in (a) were treated with rifampicin and cephlexin for 3–5 generations and analysed by flow cytometry. A total of 10,000 cells were included in each measurement