Skip to main content
Fig. 5 | BMC Microbiology

Fig. 5

From: A nutrient-dependent division antagonist is regulated post-translationally by the Clp proteases in Bacillus subtilis

Fig. 5

ClpXP targets UgtP for proteolysis in vitro independent of UDP-glucose. (a) Immunoblot of purified His-UgtP after incubation with purified ClpXP. Reactions consisting of 3 μM ClpX, 6 μM ClpP, 3 μM His-UgtP, and 5 mM ATP were incubated for 45 min at room temperature. ClpXP substrate controls are shown in Additional file 6: Figure S6. (b) In vitro ClpXP cleavage assay ± UDP-glucose or glucose-6P. The assay used 3 μM ClpX, 6 μM ClpP, 3 μM His-UgtP, 5 mM ATP, and 2 mM of either UDP-glucose or glucose-6P. α-His was used to visualize His-UgtP levels by immunoblot. Each cognate set was used to gauge relative degradation (%) as shown below (n = 3, error = SD)

Back to article page