Fig. 4

Identification of T-DNA tagging sites. a Genomic distribution of T-DNAs. hiTAIL PCR product sequences were searched against the genome database of R. glutinis ATCC 204091. b Nicking positions of the integrated T-DNAs. Histogram of nicking sites were presented based on the nicking frequency calculated from 61 LB and 196 RB flanking sequences. T-DNA border repeats were capitalized. Arrows indicate the positions of T-DNA nicking by Agrobacterium. c Size distribution of microhomology. Columns show the distribution of microhomology sizes found in this study. Black line indicated the expected sizes of microhomology based on calculation. d Classification of proteins affected by the 132 T-DNA tags