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Fig. 3 | BMC Microbiology

Fig. 3

From: Identification of novel genes in the carotenogenic and oleaginous yeast Rhodotorula toruloides through genome-wide insertional mutagenesis

Fig. 3

Southern blot analysis of R. toruloides transformants. Genomic DNA samples of 64 randomly selected transformants of pRH201 and wild-type strain R. toruloides ATCC 10657 (5 μg) were digested with PstI and separated by electrophoresis in 0.8% agarose gel. The 581 bp DNA fragment of partial hpt-3 gene amplified using oligos HptRU and HptRSL2 was used as the probe (Additional file 5: Table S3). Lane M, DIG-labeled DNA molecular size marker III (Roche Diagnosis, USA). Fragment sizes (bp): 564, 831, 947, 1375, 1584, 1904, 2027, 3530, 4268, 4973, 5148 and 21,226. WT: wild-type strain ATCC 10657

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BMC Microbiology

ISSN: 1471-2180

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