Table 3 Effect of induction points on α-IFN2b production by E. coli in MTP fermentations
Induction point | Maximum DCW | Time to achieve maximum DCW | Maximum α-IFN2b | Fermentation time | Specific yield | Volumetric productivity |
|---|---|---|---|---|---|---|
(h) | (g/L) | (h) | (μg/L) | (h) | (μg/g cell) | (μg/L/h) |
MTP | ||||||
4 | 3.05 (0.08) | 8 | 50.4 (0.31) | 10 | 16.5 | 5.04 |
6 | 3.64 (0.04) | 8 | 29.2 (0.01) | 12 | 8.0 | 2.43 |
8 | 4.37 (0.06) | 8 | 32.2 (0.04) | 14 | 7.4 | 2.30 |
10 | 5.02 (0.06) | 8 | 34.8 (0.04) | 16 | 6.9 | 2.18 |