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Table 2 Effect of inoculum size on α-IFN2b production by E. coli in MTP fermentations

From: Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli

Inoculum size Maximum DCW Time to achieve maximum DCW Maximum α-IFN2b Fermentation time Specific yield Volumetric productivity
% (v/v) (g/L) (h) (μg/L) (h) (μg/g cell) (μg/L/h)
MTP
 2 2.83 (0.01) 8 25.7 (0.01) 10 9.1 2.57
 4 2.89 (0.03) 8 31.4 (0.03) 10 10.9 3.14
 6 2.95 (0.03) 8 37.3 (0.01) 10 12.7 3.73
 8 3.10 (0.01) 8 49.5 (0.01) 10 15.9 4.95
  1. The results of maximum DCW and maximum α-IFN2b are the average of triplicate experiments. The value in bracket is the standard deviation. Specific yield and volumetric productivity are calculated with the average values. Fermentation time is the time taken from inoculation to reach a maximum concentration of α-IFN2b