From: Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli
Type of osmotic shock extraction
DCW
α-IFN2b release
Volume of sample
(g/L)
(μg/L)
(mL)
Automated
3.05 ± 0.08
49.2 ± 0.55
1
Manual
2.94 ± 0.12
48.8 ± 1.34