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Table 1 Comparison of two different methods of osmotic extraction of α-IFN2b, manual and automated microscale platform

From: Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli

Type of osmotic shock extraction

DCW

α-IFN2b release

Volume of sample

(g/L)

(μg/L)

(mL)

Automated

3.05 ± 0.08

49.2 ± 0.55

1

Manual

2.94 ± 0.12

48.8 ± 1.34

1

  1. The results of DCW and α-IFN2b release are the average of triplicate experiments