TY - JOUR AU - Tan, Joo Shun AU - Abbasiliasi, Sahar AU - Kadkhodaei, Saeid AU - Tam, Yew Joon AU - Tang, Teck-Kim AU - Lee, Yee-Ying AU - Ariff, Arbakariya B. PY - 2018 DA - 2018/01/04 TI - Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli JO - BMC Microbiology SP - 3 VL - 18 IS - 1 AB - Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation setup embedded into an automated liquid-handling system. The objective of this study was to establish an automated microscale method for upstream and downstream bioprocessing of α-IFN2b production by recombinant Escherichia coli. The extraction performance of α-IFN2b by osmotic shock using two different systems, automated microscale platform and manual extraction in MTP was compared. SN - 1471-2180 UR - https://doi.org/10.1186/s12866-017-1145-9 DO - 10.1186/s12866-017-1145-9 ID - Tan2018 ER -