Table 2 Primers used in this study
From: Isolation and characterization of HepP: a virulence-related Pseudomonas aeruginosa heparinase
Name | Sequence | Use |
|---|---|---|
hepP-For1 | GGACTGGCAAGCTGATAGGA | Analysis of hepP transcription Analysis of operon |
hepP-Rev1 | CGATACGCAAGGAAAGAGGA | Analysis of hepP transcription Analysis of operon |
zbdP-For1 | CGGGTGTTGGCTATTGATTT | Analysis of zbdP transcription |
zbdP-Rev1 | AACAATCCGTCCACGCTTAC | Analysis of zbdP transcription |
zbdP-For2 | CTTCTGAGGACAAGGCTTCG | Analysis of operon |
hepP-Rev2 | GATAAGGCTCCCAACCTTC | Analysis of operon |
zbdP-For3 | AGCCGACTATCGCTTGTGAA | Confirmation of hepP mutation |
hepP-Rev3 | GTGCTTTTCGAGCAATGGAG | Confirmation of hepP mutation |
hepP-ATG | ATGGCACTTCAAAAACTCGTCCG | Cloning hepP |
hepP-[-TGA] | ACTCGTATTTGCTGGGGTGGAATT | Cloning hepP |
Trx Forward | TTCCTCGACGCTAACCTG | Confirmation of cloning |
pBAD Reverse | GATTTAATCTGTATCAGG | Confirmation of cloning |
hepP-For2 | CGCTGGTGCAACAAGTAGAA | Analysis of hepP transcription |
hepP-Rev4 | GCGTGATACATCGGAGACAA | Analysis of hepP transcription |