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Table 1 Strains and plasmids used in this study

From: Isolation and characterization of HepP: a virulence-related Pseudomonas aeruginosa heparinase

Strains or plasmids Characteristics Reference
Pseudomonas aeruginosa
PA14 UCBPP-PA14; prototropic strain isolated from infected wound [30, 31]
PA14ΔhepP PA14/MrT7::PA14_23430–480; GmR [30]
PA14ΔzbdP PA14/MrT7::PA14_23420–118; GmR [30]
Escherichia coli
TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacΧ74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
OP50 Uracil auxotroph; feeder strain for Caenorhabditis elegans [49]
Caenorhabditis elegans
BW54 Temperature sensitive embryonic lethal strain; proliferates at 15 °C; sterile at 25 °C Caenorhabditis Genetics Center
Plasmids
pBAD/Thio-TOPO pBR322-derived expression vector; cloned genes expressed from PBAD; amino terminus HP-thioredoxin; carboxy terminus V5 epitope and 6XHis; CbR Invitrogen
pND1 pBAD/Thio-TOPO/hepP; CbR This study
  1. Cb R carbenicillin resistant, Gm R gentamincin resistant, Str R streptomycin resistant