Table 1 Strains and plasmids used in this study
From: Isolation and characterization of HepP: a virulence-related Pseudomonas aeruginosa heparinase
| Strains or plasmids | Characteristics | Reference |
|---|---|---|
| Pseudomonas aeruginosa | ||
| PA14 | UCBPP-PA14; prototropic strain isolated from infected wound | [30, 31] |
| PA14ΔhepP | PA14/MrT7::PA14_23430–480; GmR | [30] |
| PA14ΔzbdP | PA14/MrT7::PA14_23420–118; GmR | [30] |
| Escherichia coli | ||
| TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacΧ74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| OP50 | Uracil auxotroph; feeder strain for Caenorhabditis elegans | [49] |
| Caenorhabditis elegans | ||
| BW54 | Temperature sensitive embryonic lethal strain; proliferates at 15 °C; sterile at 25 °C | Caenorhabditis Genetics Center |
| Plasmids | ||
| pBAD/Thio-TOPO | pBR322-derived expression vector; cloned genes expressed from PBAD; amino terminus HP-thioredoxin; carboxy terminus V5 epitope and 6XHis; CbR | Invitrogen |
| pND1 | pBAD/Thio-TOPO/hepP; CbR | This study |
