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Fig. 8 | BMC Microbiology

Fig. 8

From: Isolation and characterization of HepP: a virulence-related Pseudomonas aeruginosa heparinase

Fig. 8

The hepP gene is transcribed in PA14ΔzbdP . RNA obtained from PA14ΔzbdP, and PA14ΔhepP grown in LB broth was reverse-transcribed and the cDNA used as templates in PCR reactions as indicated on the figure. Primer pairs were designed to detect internal fragments downstream of the transposon insertion in zbdP (zbdP-For1/zbdP-Rev1) or hepP (hepP-For2/hepP-Rev4). Primer pairs used in each reaction are indicated on the figure. PCR products were separated on a 0.75% agarose gel and stained with Gelstar. Lanes: MSS, molecular size standards; 1, no-template control; 2, 201-bp hepP product; 3, no zbdP product; 4, no-template control; 5, 219-bp zbdP product; 6, no hepP product

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