Fig. 2

The genes zbdP and hepP constitute an operon. a Diagram of the location of zbdP and hepP on the PA14 chromosome. Relative size, spacing, and direction of transcription (wavy arrow) are indicated. Location of primer pairs (zbdP-F1/hepP-R1 and hepP-F1/hepP-R2) used to determine transcripts is shown by arrows. The expected sizes of the PCR products are specified on the diagram. b PCR products obtained from RT-PCR experiments. PA14 was grown in LB broth, pelleted, and the RNA extracted. RT-PCR was used to obtain cDNA, which served as a template in PCR reactions with the primer pairs indicated in a. PCR products were separated on a 0.75% agarose gel and stained with Gelstar. Lanes: 1) 1 kb DNA ladder; 2) no template control; 3) product from primer pair zbdP-F1/hepP-R1; 4) hepP-F1/hepP-R2 primer pair (positive control) (Table 2)