Table 2 Primers designed in this study
From: The distinctive cell division interactome of Neisseria gonorrhoeae
Primer name | Sequences (5′-3′) | |
|---|---|---|
P1 | FtsA-reBamHI | GCGCGGATCCTCAGAGGTTGTTTTCAATCC |
P2 | FtsA-fwSalI | GCGCGTCGACCATGGAACAGCAGAAAAGATAC |
P3 | fwSalI-ftsK | GCGCGTCGACCATGTTTTGGATAGTTTTGATCGTTAT |
P4 | reBamHI-ftsK | CGCGGGATCCTCAAGCATTGTCCAAGGGGACGAG |
P5 | fwSalI-ftsQ | GCGCGTCGACCATGTGGGATAATGCCGAAGCGATG |
P6 | reBamHI-ftsQ | CGCGGGATCCCTATTCTTCGGATTCTTTTTCGGG |
P7 | fwSalI-ftsI | GCGCGTCGACCATGTTGATTAAAAGCGAATATAAGCC |
P8 | reBamHI-ftsI | CGCGGGATCCTTAAGACGGTGTTTTGACGGCTGC |
P9 | fwSalI-ftsW | GCGCGTCGACCATGAAGATTTCGGAAGTATTGGTAAA |
P10 | reBamHI-ftsW | CGCGGGATCCTTACTCCACCCGGTAACCGCGCAT |
P11 | fwSalI-ftsN | GCGCGTCGACCATGTTTATGAACAAATTTTCCCAATC |
P12 | reBamHI-ftsN | CGCGGGATCCTTATTTGCCTTCAATCGCACGGAT |
P13 | fwBglII-ZipA | GCGCGAGATCTGATGATTTACATCGTACTGTTCCTC |
P14 | reBamHI-ZipA | CGCGGGATCCTTATGAAAACAGGCGCAGGGC |
P15 | FtsA-reEcoRI-pET30a | ATATCGAATTCTCAGAGGTTGTTTTCAATCCACC |
P16 | FtsA-fwBglII-pET30a | AGCCCAGATCTGATGGAACAGCAGAAAAGATACATC |
P17 | fwBglII-FtsQ- pET30a | AGCCCAGATCTGATGTGGGATAATGCCGAAGCGATG |
P18 | reEcoRI-ftsQ- pET30a | ATATCGAATTCCTATTCTTCGGATTCTTTTTCGGG |
P19 | FtsZ-fwBgl II-pET30a | AGCCCAGATCTGATGGAATTTGTTTACGACGTGGCA |
P20 | FtsZ-ReEcoRI-pET30a | AGCCCGAATTCTTATTTGTCTGAATTGTGTTGACG |
P21 | fwFtsA-BamHI-GST | CGCGGGATCCATGGAACAGCAGAAAAGATACATC |
P22 | fwEcoRI-FtsN | GACGAATTCATGTTTATGAACAAATTTTCCCAATCC |
P23 | reXhoI-FtsN | GACCTCGAGTTATTTGCCTTCAATCGCACG |