Fig. 1
From: The distinctive cell division interactome of Neisseria gonorrhoeae
Interactions of FtsANg with FtsQNg, FtsNNg and FtsZNg by GST pull-down. a GST pull down between His-FtsQNg and GST-FtsANg. Lane 1: His-FtsQNg input; Lane 2: GST-FtsANg and His-FtsQNg mixture; Lane 3: GST and His-FtsQNg mixture; b GST pull down between His-FtsANg and GST-FtsNNg. Lane 1: His-FtsANg input; Lane 2: GST-FtsNNg and His-FtsANg mixture, GST-FtsNNg was loaded with GST and GST-FtsNNg degradation products; Lane 3: GST and His-FtsANg mixture; c GST pull down between His-FtsZNg and GST-FtsANg. Lane 1: His-FtsZNg input; Lane 2: GST-FtsANg and His-FtsZNg mixture; Lane 3: GST and His-FtsZNg mixture; His-tagged fusion proteins were visualized using anti-6 × His antibody; GST and GST-tagged fusion proteins were visualized using anti-GST antibody