Investigation on the differentiation of two Ustilago esculenta strains - implications of a relationship with the host phenotypes appearing in the fields

Table 1 Comparision of the mating response and the length of filaments in presence of distinct nitrogen or carbon sources in vitro

All the hyphal length data were detected three days after mating assays on the basic solid medium with different kind of nitrogen or carbon sources. The basic medium with 20 mmol/L KNO3 and 50 mmol/L sucrose was prepared as the blank control (CK). The superscript letters showed significant differences of the hyphal length at P < 0.05 level, according to One-Way ANOVA analysis with the Duncan method. Integrated mating ability was analyzed by the hyphal length and amount (see Additional file 7), comparing with the mating status of MT strains on CK medium, e.g., a significant decrease in hyphal length from the contrast (1164.4 ± 234.0) means shorter and a similar or longer hyphal length from the contrast means normal. Therein, “-” means no hyphae formation; “+” means less hyphae or shorter hyphal length, without white aerial hyphae; “++” means normal hyphal amount and length, without white aerial hyphae; “+++” means normal hyphal amount and length, with white aerial hyphae observed

ISSN: 1471-2180