Fig. 7
From: 6S RNA plays a role in recovery from nitrogen depletion in Synechocystis sp. PCC 6803
Relative content of different sigma factors (σ) in RNAP holoenzyme (left panel; a, c, e, g) and in the soluble protein fraction (right panel; b, d, f, h) of WT-RNAP-HIS (WT) and ΔssaA-RNAP-HIS mutant (ΔssaA) strain under standard conditions (Std), under nitrogen deficiency for 6 days (t = 0 h + N) and during recovery (t = 1 h + N, t = 4 h + N and t = 22 h + N). Equal amounts of collected RNAP complexes or soluble proteins were separated on SDS-PAGE, group 1 σ factor SigA (a, b) and group 2 σ factors SigB (c, d), SigC (e, f) and SigE (g, h) were detected by immunoblot analysis. The σ factor content was normalized to α or β RNAP core subunit. For each σ factor the value of the WT-RNAP-HIS strain at standard conditions (Std) was set to 100 and the other samples were compared respectively. Three independent biological replicates were analyzed and the error bars represent the standard error