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Fig. 3 | BMC Microbiology

Fig. 3

From: Expression and evolutionary patterns of mycobacteriophage D29 and its temperate close relatives

Fig. 3

Transcriptomic analysis of L5. a. Strand-specific RNAseq analysis of the L5 thermo-inducible lysogen (green), and 30 min (blue) and 150 min (red) after induction; a map of the L5 genome is shown at the bottom. At the left are scale maxima as well as indication of top or bottom strand. b. The top strand of the 150 min RNAseq sample indicating the beginning of transcription from Pright. c. Whole genome alignment from Fig. 1b-e enlarged to highlight the Pright locus at the left end of the genome, with nucleotide shading as in Fig. 2d. A stoperator site, the beginning of the RNAseq expression data, and the translation start site of gene 1 are labeled. d. Putative terminator structures ending rightwards late transcription (e.g. in the L5 3233 region), and the alignment of the L5, D29 and StarStuff terminator sequences is shown below. e. The RNAseq profile at 30 min post induction at the extreme right end of the L5 genome. About 30 bp are under-represented between the beginning of the reads and Pleft, which was mapped previously using S1 nuclease protection and primer extension [19]

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