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Fig. 2 | BMC Microbiology

Fig. 2

From: Expression and evolutionary patterns of mycobacteriophage D29 and its temperate close relatives

Fig. 2

StarStuff lysogen is immune to D29 superinfection. a. Comparison of plaques from each phage (D29, StarStuff, L5 and Bxb1) plated on wild type M. smegmatis mc2155. D29 has large, clear plaques, indicative of its lytic lifestyle; whereas, the temperate phage StarStuff plaques are smaller and more turbid. Both L5 and Bxb1 are also temperate phages. b. Each plate represents a lawn of the bacterial strain indicated at the top of each plate. Phages (names on left) were ten-fold serially diluted and spotted onto each lawn. The StarStuff lysogen is immune to infection by D29, due to superinfection immunity. L5, StarStuff and D29 can infect M. tuberculosis mc27000. c. Amino acid alignment of the immunity repressor from Subcluster A2 phages in Fig. 1a, including the predicted truncated D29 repressor. Alignment positions with variant residues are shaded grey. The predicted helix-turn-helix DNA-binding domain is labeled. Amino acid positions are labeled to the right. The StarStuff, Pomar16 and Kerberos repressors share 78–79% amino acid identity with the L5 repressor. d. Whole genome alignment from Fig. 1b-e enlarged to highlight the Pleft locus at the right end of the genome. Variable nucleotide positions are shaded grey as in panel C. Empirically identified features, such as the operator site, two stoperator sites, and the transcription start site (TSS) are labeled, along with the predicted −10 and −35 promoter elements. Genome coordinates are labeled to the right

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