Classification of a new phytoplasmas subgroup 16SrII-W associated with Crotalaria witches’ broom diseases in Oman based on multigene sequence analysis

Table 2 Primers used for PCR amplification and sequencing of the 16S rRNA, secA, imp, tuf, and SAP11 genes of the phytoplasma

Primer Sets	Location	PCR product size	Reaction	Sequence (5′ to 3′)
SecA-II-F1/SecA-II-R1	secA gene	2141 bp	Direct PCR	AAAGATGAAGATTTTCCTAAAGA/TCCATATCATTTATATGACGTTGA
SecA-II-F1/SecA-II-R4	secA gene	1511 bp	Semi-Nested PCR	AAAGATGAAGATTTTCCTAAAGA/ACAAAAAATTTAGTATAACCAGGATC
IMP-II-F1/IMP-II-R1	imp gene	786 bp	Direct PCR	GTTATAATTGAAGGCGATATTG/ATAGAGGAGAAGAAAAAGTTTCT
IMP-II-F2/IMP-II-R1	imp gene	717 bp	Semi-Nested PCR	GATCATATTTGGTTTATAGGAG/ATAGAGGAGAAGAAAAAGTTTCT
TUF-II-F1/TUF-II-R1	tuf gene	1490 bp	Direct PCR	GCTTTTGTTCCTTTAGCAGAA/AGACTATACACTAGTCTTCTT
TUF-II-F2/TUF-II-R1	tuf gene	1094 bp	Semi-Nested PCR	CGCAAAGATATTAAAACTTTAG/AGACTATACACTAGTCTTCTT
SAP11-IID-F1/SAP11-IID-R1	SAP11 gene	820 bp	Direct PCR	CGGCAAAATAAAAGTTCAAATCA/AATCGAAACCAACCAACTTATAG
SAP11-IID-F2/SAP11-IID-R2	SAP11 gene	465 bp	Nested PCR	TTCTCAATTAAACGAACTCTACG/AAAAAGACCCTTCAGAAAGGGTC
SAP11-WBDL-F1/SAP11-WBDL-R1	SAP11 gene	1050 bp	Direct PCR	CTTCAGCCACAAATAGAATCTTT/CAAATACAAATCGCTGCATAAA
SAP11-WBDL-F2/SAP11-WBDL-R2	SAP11 gene	550 bp	Nested PCR	TTCCTTTTATGAAATCACCTCAG/GCGCATATTATTAAACTCCTTT

ISSN: 1471-2180