Table 2 Performance of LBJMR medium

Isolates screened

Colistin-resistant Gram-negatives:

- Enterobacteriaceae, including those harboring the mcr-1 gene

- Non-fermentative Gram-negative colistin-resistant strains, including those involves in cystic fibrosis samples

Vancomycin-resistant Gram positives, including Enterococci

Aspect of colonies

Yellow on purple agar: 2–3 mm for Enterobacteriaceae, 0.1–1 mm for Enterococci

Incubation

Aerobic atmosphere, 37 °C, 24H (sterile at 48H)

Culture of samples

Direct on LBJMR, no previous decontamination

Isolates analysis

Colonies can be picked directly from primary cultures on LBJMR for analysis:

- MALDI-TOF identification

- Antibiotic Susceptibility testing

- PCR screening for resistance genes.

Avoid contamination

- Inhibition of Proteus swarming

- Inhibition of yeast possible by adding amphotericin B