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Table 1 Detection limits of targeted bacteria on LBJMR and SuperPolymyxin culture media

From: LBJMR medium: a new polyvalent culture medium for isolating and selecting vancomycin and colistin-resistant bacteria

Bacterial strains Detection limits on culture media (CFU):
Gram-negatives   CT MIC LBJMR SuperPolymyxin
Colistin-resistant Enterobacteriaceae E. coli SE65 4 101 101
E. coli 117R 4 101 101
E. coli 1R 4 101 101
E. coli 1R 2104 4 101 101
E. coli 44A 4 101 101
E. coli 6R 4 101 101
E. coli 85R 4 101 101
E. coli 95R 4 101 101
E. coli 96R 4 101 101
E. coli 134R 3 101 101
E. coli 143R 3 101 101
E. coli LH121 16 101 101
E. coli LH140 1 12 101 101
E. coli LH257 12 101 101
E. coli LH57 1 8 101 101
E. coli LH1 6 101 101
E. coli LH30 6 101 101
E. coli TH214 6 101 101
E. coli TH99 4 101 101
E. coli 235 4 101 101
E. coli P6 6 101 101
E. coli P10 4 101 101
E. coli P17 4 101 101
E. coli FHA1022 12 101 101
E. coli FHM193 12 101 101
E. coli FHA1134 12 101 101
E. coli NH945 12 101 101
E. coli TH176 6 101 101
K. pneumoniae FHA60 8 101 101
K. pneumoniae FHM128 4 101 101
K. pneumoniae 119R 3 101 101
K. pneumoniae LH131 6 32 101 101
K. pneumoniae LH61 7 24 101 101
K. pneumoniae LH17 12 101 101
K. pneumoniae LH92 12 101 101
K. pneumoniae LB16 32 101 101
P. mirabilis FH112 >256 101 101
P. vulgaris PV148 >256 101 101
P. alcalifaciens TH44 >256 101 101
M. morganii FM102 >256 101 101
S. marcescens E13 128 101 101
Others colistin-resistant B. cepacia FHM-BC1 64 103 105
B. cepacia FHM-BC2 >256 101 101
A. xylosoxidans FHM-AX 3 101 >105
S. maltophilia FHM-SM 12 103 >105
I. limosus FHM-IL >256 104 104
P. pulmonicola FHM-PP >256 101 103
S. putrefaciens FHM-SP NC 103 >105
O. anthropi FHM-OA NC 102 >105
P. aeruginosa FHM-PACOLR1 >256 101 101
A. baumannii ABIsac_ColiR 8 102 101
Colistin-susceptible E. asburiae P113 0,19 >105 >105
E. cloacae NH151 0,50 >105 >105
E. cloacae NH74 0,38 >105 >105
K. pneumoniae CIP 82.91 0,125 >105 >105
K. pneumoniae LB2 0,128 >105 >105
K. pneumoniae TH20S 0,125 >105 >105
K. pneumoniae TH28S 0,125 >105 >105
Proteus vulgaris P100 0,94 >105 >105
S. enterica 108R 1 >105 >105
S. enterica 122R 0,5 >105 >105
E. coli ATCC 25922 CIP 76.24 0,094 >105 >105
E. coli 161 0,094 >105 >105
E. coli 169 0,094 >105 >105
E. coli FHM88S 0,125 >105 >105
E. coli TH134S 0,094 >105 >105
E. coli LH53S 0,094 >105 >105
E. coli LH165S 0,074 >105 >105
E. coli TH77S 0,064 >105 >105
E. coli 282S 0,074 >105 >105
E. coli FHM19S 0,125 >105 >105
P. aeruginosa FHM-PA4 2 >105 >105
P. aeruginosa FHM-PA5 0,50 >105 >105
P. aeruginosa FHM-PA6 0,38 >105 >105
S. xianemensis 111P 0,125 >105 >105
S. xianemensis 111A 0,094 >105 >105
A. nosocomialis ABG13S 0,064 >105 >105
A. pitti MK 0,125 >105 >105
Gram-positives   VCN MIC   
Vancomycin-resistant E. faecium DSM17050a >256 101 >105
E. faecium DSM13590a >256 101 >105
E. faecium DSM25698a >256 102 >105
E. faecium DSM25697a >256 101 >105
E. faecium FHMVRE1a >256 101 >105
E. faecium FHMVRE2a >256 101 >105
E. faecium VRE 24 103 >105
E. faecalis JH2–2: Tn 1549b 64 102 >105
W. cibaria P18A >256 101 >105
W. cibaria P18B >256 101 >105
L. lactis P18C >256 101 >105
Vancomycin-susceptible S. aureus CF_Marseille 1 >105 >105
E. faecium TH26 0,75 >105 >105
E. faecium 349 0,75 >105 >105
E. faecium TH12 2 >105 >105
E. faecium LH165 0,75 >105 >105
E. faecium Al7 0,75 >105 >105
E. faecium TH43 0,75 >105 >105
E. faecium TH95 0,75 >105 >105
E. faecium 282 0,75 >105 >105
E. faecalis JH2–2: C2 1,5 >105 >105
E. faecalis JH2–2S 1,5 >105 >105
E. gallinarum SE3 1,5 >105 >105
E. casseliflavus SE3 1,5 >105 >105
E. hirae LH111 1 >105 >105
  1. MIC of colistin (CT) and vancomycin (VCN) are given in μg/mL. Enterobacteriaceae with mcr-1 gene are in bold font
  2. Colistin-resistant strains with a mutation on another gene are indicated as follow: 1 phoP E375K, 2 pmrB A159V, 3 pmrB P7-Q12(del 6aa), 4 pmrB T156 K, 5 pmrB I92 insertion, 6 mgrB Stop, 7 mgrB Sub, 8 pmrB T157P. Enterococci strains with vancomycin-resistant genes are indicated as follows: a vanA and b vanB
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BMC Microbiology

ISSN: 1471-2180

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