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Table 1 Bacterial strains and plasmids used in this study

From: Characterization of the inner membrane protein BB0173 from Borrelia burgdorferi

Bacterial strain or plasmid

Genotype

Source

Borrelia burgdorferi B31A3

cp9, wild type

Rocky Mountain Labs [59]

E. coli strains

 OneShot Top10

Cloning host; F mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL(Strr) endA1 nupG

Invitrogen

 Rosetta (DE3)pLysS

Expression host; F ompT hsdS B(rB mB ) gal dcm (DE3) pLysSRARE (CamR)

Novagen

Plasmids

 pCS1–5

pCR2.1(bb0173 T )

This study

 pCS1–9

pET23a(bb0173 T )

This study