From: Characterization of the inner membrane protein BB0173 from Borrelia burgdorferi
Primer pair | RS | Sequence (5’➔3′) | Application |
---|---|---|---|
bb0173T-NdeI-F | NdeI | ACG CCA TAT GGC TTT AGC AGG TCC TTC | Amplification of bb0173 for cloning into pCR2.1 and expression vector pET23a |
bb0173T-XhoI-R | XhoI | ACG CCT CGA GTA GTA TCT CTT TTA AG | |
bb0173cDNA-F(nt249–272) | GAA GAT GAT ACA TCT TAG TGC TGG | Amplification of bb0173 cDNA from RNA | |
bb0173cDNA-R(nt725–749) | CTT CCC TGA TAA AAT TTT CCA GAT | ||
bb0173TM1-SpeI-F | SpeI | ACG CAC TAG TGG AGG ACC AGG AAA TGA GCC TTT ATA TTT G | Amplification of the first putative bb0173 transmembrane sequence in frame with the Lep construct |
bb0173TM1-KpnI-R | KpnI | ACG CGG TAC CCC TCC TGG TCC CTT TAT CTT GCC TCC TCT | |
bb0173TM1–2-SpeI-F | SpeI | ACG CAC TAG TGG AGG ACC AGG AGA TTA TAG ATT AA TTT G | Amplification of the second putative bb0173 transmembrane sequence in frame with the Lep construct |
bb0173TM2-KpnI-R | KpnI | ACG CGG TAC CCC TCC TGG TCC AAC TGA AGG ACC TGC TAA AGC | |
bb0173TM2b-SpeI-F | SpeI | ACG CAC TAG TGG AGG ACC AGG ACT AGA TGA TAT TTA TAT TAT G | Amplification of the third putative bb0173 transmembrane sequence in frame with the Lep construct |
bb0173TM2b-KpnI-R | KpnI | AGC CGG TAC CCC TCC TGG TCC AGC CTC AGA ATG CTT TAA ATG | |
bb0173TM3-SpeI-F | SpeI | ACG CAC TAG TGG AGG ACC AGG AGA TAT TTA TAA AGA ATT TTT AG | Amplification of the fourth putative bb0173 transmembrane sequence in frame with the Lep construct |
bb0173TM3-KpnI-R | KpnI | ACG CGG TAC CCC TCC TGG TCC CTC TTT TAA GAA AAT TTT TG | |
bb0173-NcoI-F.1 | NcoI | ACG CCC ATG GAT GTT AAC ATT TAA TGA G | Common amplification start site for truncated insertion constructs |
T7BB173 | ATA GTA TAA TAC GAC TCA CTA TAG GAA ACC ACC ATG GGC ATG TTA ACA TTT AAT G | Amplification of BB173 with T7 promoter | |
TM1 NST BB173 | TTA TCA GGA CAT CAT GGT GCT GTT ATA ATC CTT AAG TTT TAA | Amplification of BB173 56mer adding C-tag NST | |
TM1 QST BB173 | TTA TCA GGA CAT CAT GGT GCT CTG ATA ATC CTT AAG TTT TAA | Amplification of BB173 56mer adding C-tag QSTMMS | |
TM2 NST BB173 | TTA TCA GGA CAT CAT GGT GCT GTT AAT ATC ATC TAG CTT TT | Amplification of BB173 162mer adding C-tag NST | |
TM2 QST BB173 | TTA TCA GGA CAT CAT GGT GCT CTG AAT ATC ATC TAG CTT TT | Amplification of BB173 162mer adding C-tag QSTMMS | |
TM3 NST BB173 | TTA TCA GGA CAT CAT GGT GCT ATT AAG CAT ACT AGG ATC | Amplification of BB173 278mer adding C-tag NST | |
TM3 QST BB173 | TTA TCA GGA CAT CAT GGT GCT CTG AAG CAT ACT AGG ATC | Amplification of BB173 278mer adding C-tag QSTMMS | |
TM 4 NST BB173 | TTA TCA GGA CAT CAT GGT GCT GTT CCC GGA CAT GCT GCC CAT TAG TAT CTC TTT TAA GAA | Amplification of BB173 341-mer (full length) adding C-tag NST | |
TM 4 QST BB173 | TTA TCA GGA CAT CAT GGT GCT CTG CCC GGA CAT GCT GCC CAT TAG TAT CTC TTT TAA GA | Amplification of BB173 341-mer (full length) adding C-tag QSTMMS | |
TM2 NST BB173 | TTA TCA GGA CAT CAT GGT GCT GTT AAT ATC ATC TAG CTT TT | Amplification of BB173 162mer adding C-tag NST | |
TM2 QST BB173 | TTA TCA GGA CAT CAT GGT GCT CTG AAT ATC ATC TAG CTT TT | Amplification of BB173 162mer adding C-tag QSTMMS | |
TM3 NST BB173 | TTA TCA GGA CAT CAT GGT GCT ATT AAG CAT ACT AGG ATC | Amplification of BB173 278mer adding C-tag NST | |
TM3 QST BB173 | TTA TCA GGA CAT CAT GGT GCT CTG AAG CAT ACT AGG ATC | Amplification of BB173 278mer adding C-tag QSTMMS | |
bb0170 qPCR F | GTT AAA CCG ATT CCT GGA GAG | Amplification of bb0170 from cDNA for expression studies | |
bb0170 qPCR R | CAG CCA AAA CTT GAT GCT GC | ||
bb0171 qPCR F | AAA TCC ATG TCT TTA ATG | Amplification of bb0171 from cDNA for expression studies | |
bb0171 qPCR R | AAC CCT CTC AAG ATT TTC | ||
bb0172 qPCR F | TAT GGG GAC AAT TCT TAT ATT CAA | Amplification of bb0172 from cDNA for expression studies | |
bb0172 qPCR R | CAA TCC CAA CCA CAA AAC TTT CCA | ||
bb0173 qPCR F | ATT TAA TGA GCC TTT ATA TTT GTT TTT A | Amplification of bb0173 from cDNA for expression studies | |
bb0173 qPCR R | GAT CCA TAA TAT AAA TAT CAT CTA GCT T | ||
bb0174 qPCR F | GAT GGT GAA GAG TTT TCC | Amplification of bb0174 from cDNA for expression studies | |
bb0174 qPCR R | TTC TGT TGT AGT GAT TGC | ||
bb0175 qPCR F | TTT CAT GAG TTT AGG CCG | Amplification of bb0175 from cDNA for expression studies | |
bb0175 qPCR R | TGT TGA CTT GCT AAA CCC | ||
bb0176 qPCR F | TTA CTT GAA GGG GTT CCG | Amplification of bb0176 from cDNA for expression studies | |
bb0176 qPCR R | ATC CCT TTC ACG GAG TGC | ||
rpoN qPCR F | TTG TAC CCC ATT CGC ATT TT | Amplification of rpoN from cDNA for expression studies | |
rpoN qPCR R | GTG AAA ACC CCC AAA AAC AA | ||
rpoS qPCR F | TTG GGC GAT TTT TCT TCT TC | Amplification of rpoS from cDNA for expression studies | |
rpoS qPCR R | TGC GGG TAA AGG GTT AAA AA | ||
rrp2 qPCR F | TGT AGC TTC TCC CCC AAC AC | Amplification of rrp2 from cDNA for expression studies | |
rrp2 qPCR R | TTT TGG CCA TGA AAA AGG AG | ||
bb0420QF | TGG CAA GTC GAA TAC CCT CT | Amplification of hpk1 from cDNA for expression studies; From Rogers et al., 2009 [34]. | |
bb0420QR | TGT TCG ATT TTA TTG GGA TGC | ||
bb0419F-RT | TTG AGG TTG CAA CAA ATG GA | Amplification of rrp1 from cDNA for expression studies; From Rogers et al., 2009 [34]. | |
bb0410R-RT | CGG GAT CGC TTT TTA GCT TT |