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Fig. 7 | BMC Microbiology

Fig. 7

From: Characterization of the inner membrane protein BB0173 from Borrelia burgdorferi

Fig. 7

Protection of BB0173 from protease degradation. Surface proteins of B. burgdorferi are degraded by serine protease Proteinase K (PK). Whole cell lysates were treated with doses ranging from 0 to 200 μg/mL PK prior to separation using SDS-12% PAGE. Gels were either visualized using Coomassie blue staining (a) or transferred to a PVDF membrane and probed with antibodies (b). BB0173 was detected using anti-BB0173pep and anti-chicken HRP-conjugated antibody. Controls for PK mediated degradation and cell integrity during treatment included intercellular protein BosR and periplasmic protein FlaB, as well as outer membrane proteins OspC, VlsE, and P66

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