Fig. 6
From: Characterization of the inner membrane protein BB0173 from Borrelia burgdorferi
Localization of BB0173 to the aqueous and inner membrane fractions after treatment with detergent. B. burgdorferi cells disrupted using the detergent Triton X-114 separated into three distinct fractions, the aqueous (AQ), protoplasmic cylinders (PC), and detergent (DT) phases. The phases were separated using SDS-12% PAGE and either stained using Silver Stain Plus (Biorad, Hercules, CA) (a) or were transferred to a PVDF membrane and probed using anti-BB0173T and a secondary anti-chicken HRP-conjugated antibody Lane 1 is B. burgdorferi whole cell lysate. Lane 2 is AQ, Lane 3 is PC, and Lane 4 is DT (b). Controls for outer membrane and inner membrane proteins were OspC and FlaB