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Fig. 2 | BMC Microbiology

Fig. 2

From: Characterization of the inner membrane protein BB0173 from Borrelia burgdorferi

Fig. 2

Expression of bb0173 cDNA upon temperature shift. B. burgdorferi B31A3 strain was grown under unfed tick conditions (RT/pH 7.6) to late log phase then shifted to fed tick conditions (37 °C/pH 6.8) before collection of mRNA. The purified mRNA was reverse transcribed to cDNA, and PCR was performed to detect bb0173, flaB, p66, and ospC. Water was used as a negative control (−). a RNA samples were tested for DNA contamination in lanes 3 and 6. Genomic DNA was run in lanes 2 and 5 and served as the positive control. In lanes 1 and 4, cDNA samples were loaded. To confirm functionality of primers, a second genomic DNA sample was applied in lane 7. b The same shifting conditions were used to generate DNA samples as previously. cDNA samples are in lanes 1 and 3, RNA in lanes 2 and 4, and genomic DNA is labeled as (+). Negative control is water, as above. On the left of the figure, the DNA ladder is shown and sizes are denoted in basepairs

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