Fig. 3
From: Phosphate signaling through alternate conformations of the PstSCAB phosphate transporter
Pi uptake in the pstB mutant strains. Cultures of BW26337 cells with pRR48, p48SCABU, p48SCAB(Q160K)U or p48SCAB(E179Q)U were grown overnight in MOPS LoPi medium. After starving them of Pi and then adding K2HPO4, Pi uptake was measured. Each strain was assayed using two biological replicates in duplicate. Error bars represent ± the standard deviation. *, P < 0.05 between the p48SCABU and p48SCAB(Q160K)U strains and **, P < 0.01 between the p48SCABU and p48SCAB(E179Q)U strains, as determined by a two-tailed Student t test