Table 1 Bacterial strains and plasmids used in this study

 M93Sm

Spontaneous Sm^r mutant of M93 (serotype O2a)

[47]

 XM420

Sm^r Cm^r; icd insertional mutant

This study

 ES422

Sm^r; Restored icd strain

This study

 XM440

Sm^r Cm^r; sucA insertional mutant

This study

 XM450

Sm^r Cm^r; sucC insertional mutant

This study

 XM460

Sm^r Cm^r; sdhC insertional mutant

This study

 XM470

Sm^r Cm^r; fumA insertional mutant

This study

 XM410

Sm^r Cm^r; mdh insertional mutant

This study

 XM430

Sm^r Cm^r; cra insertional mutant

This study

 SM10

thi thr leu tonA lacY supE recA RP4–2-Tc::Mu::Km (λ pir)

[48]

 S100

Km^r; Sm10 containing plasmid pNQ705–1

[49]

 Q420

Km^r Cm^r; Sm10 containing plasmid pNQ705-icd

This study

 Q440

Km^r Cm^r; Sm10 containing plasmid pNQ705-sucA

This study

 Q450

Km^r Cm^r; Sm10 containing plasmid pNQ705-sucC

This study

 Q460

Km^r Cm^r; Sm10 containing plasmid pNQ705-sdhC

This study

 Q470

Km^r Cm^r; Sm10 containing plasmid pNQ705-fumA

This study

 Q410

Km^r Cm^r; Sm10 containing plasmid pNQ705-mdh

This study

 Q430

Km^r Cm^r; Sm10 containing plasmid pNQ705-cra

This study

 pNQ705–1

Cm^r; suicide vector with R6K origin

[49]

 pNQ705-icd

Cm^r; For icd insertional mutant

This study

 pNQ705-sucA

Cm^r; For sucA insertional mutant

This study

 pNQ705-sucC

Cm^r; For sucC insertional mutant

This study

 pNQ705-sdhC

Cm^r; For sdhC insertional mutant

This study

 pNQ705-fumA

Cm^r; For fumA insertional mutant

This study

 pNQ705-mdh

Cm^r; For mdh insertional mutant

This study

 pNQ705-cra

Cm^r; For cra insertional mutant

This study