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Table 1 Bacterial strains and plasmids used in this study

From: Isocitrate dehydrogenase mutation in Vibrio anguillarum results in virulence attenuation and immunoprotection in rainbow trout (Oncorhynchus mykiss)

Strain or plasmid Description Reference
V. anguillarum strains
 M93Sm Spontaneous Smr mutant of M93 (serotype O2a) [47]
 XM420 Smr Cmr; icd insertional mutant This study
 ES422 Smr; Restored icd strain This study
 XM440 Smr Cmr; sucA insertional mutant This study
 XM450 Smr Cmr; sucC insertional mutant This study
 XM460 Smr Cmr; sdhC insertional mutant This study
 XM470 Smr Cmr; fumA insertional mutant This study
 XM410 Smr Cmr; mdh insertional mutant This study
 XM430 Smr Cmr; cra insertional mutant This study
E. coli strains
 SM10 thi thr leu tonA lacY supE recA RP4–2-Tc::Mu::Km (λ pir) [48]
 S100 Kmr; Sm10 containing plasmid pNQ705–1 [49]
 Q420 Kmr Cmr; Sm10 containing plasmid pNQ705-icd This study
 Q440 Kmr Cmr; Sm10 containing plasmid pNQ705-sucA This study
 Q450 Kmr Cmr; Sm10 containing plasmid pNQ705-sucC This study
 Q460 Kmr Cmr; Sm10 containing plasmid pNQ705-sdhC This study
 Q470 Kmr Cmr; Sm10 containing plasmid pNQ705-fumA This study
 Q410 Kmr Cmr; Sm10 containing plasmid pNQ705-mdh This study
 Q430 Kmr Cmr; Sm10 containing plasmid pNQ705-cra This study
Plasmid
 pNQ705–1 Cmr; suicide vector with R6K origin [49]
 pNQ705-icd Cmr; For icd insertional mutant This study
 pNQ705-sucA Cmr; For sucA insertional mutant This study
 pNQ705-sucC Cmr; For sucC insertional mutant This study
 pNQ705-sdhC Cmr; For sdhC insertional mutant This study
 pNQ705-fumA Cmr; For fumA insertional mutant This study
 pNQ705-mdh Cmr; For mdh insertional mutant This study
 pNQ705-cra Cmr; For cra insertional mutant This study