Fig. 7
Growth of V. anguillarum WT (M93Sm) and the icd mutant under various conditions. a Final cell densities (OD600) of V. anguillarum strains after 24 h of growth in 3M plus 0.15% glucose supplemented with or without 5.9 mM glutamate. b Growth curves of V. anguillarum M93Sm (black) and the icd mutant (blue) in LB20 (dashed lines) or LB20 supplemented with 118 mM glutamate (solid lines). Statistical analysis was based on data at 24 h. c Final cell densities (OD600) of V. anguillarum M93Sm and icd mutant strains grown in LB20 supplemented with decreasing amounts of glutamate. In each experiment cells grown overnight in LB20 were washed in NSS and used to inoculate the appropriate media. Cultures were incubated at 27 °C in a shaking water bath (200 rpm) and at various time points after inoculation samples were taken for determination of optical density at 600 nm (OD600). Different letters indicate statistical significance among groups (p < 0.05). Error bars represent 1 standard deviation