BMC Microbiology

Table 4 Detection results of the multiplex real-time PCR on the spiked food samples that inoculated with different concentations of E. coli O157:H7 and enriched with different incubation time

From: Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli

Target gene	Food matrice	Incubation time (h)
	CFU/gram	0	4		8		12		24
	Beef		C_T ^a	±SD	C_T	±SD	C_T	±SD	C_T	±SD
Z3276	80	UD^b	34.50	0.29	28.43	0.07	27.98	0.10	27.98	0.10
Z3276	800	UD	30.51	0.38	24.54	0.10	24.00	0.04	24.00	0.04
stx1	80	UD	27.54	0.19	21.73	0.22	20.84	0.25	19.06	0.36
stx1	800	UD	24.45	0.22	18.51	0.14	17.46	0.03	18.48	0.02
stx2	80	UD	31.34	0.10	25.47	0.02	25.03	0.04	23.63	0.02
stx2	800	UD	27.93	0.34	21.68	0.03	21.33	0.06	22.52	0.05
IAC	200 copies^c	28.88	27.72		21.96		21.09		19.59
IAC	Spinach		C_T	±SD	C_T	±SD	C_T	±SD	C_T	±SD
Z3276	80	UD	33.92	0.04	24.32	0.26	22.86	0.15	24.26	0.09
Z3276	800	UD	30.91	0.05	23.04	0.11	23.08	0.08	23.12	0.07
stx1	80	UD	27.63	0.16	17.32	0.15	15.74	0.13	16.87	0.11
stx1	800	UD	25.04	0.06	16.57	0.12	16.25	0.15	16.37	0.10
stx2	80	UD	30.88	0.06	21.21	0.07	20.10	0.06	21.64	0.07
stx2	800	UD	28.19	0.14	20.05	0.15	20.58	0.05	20.74	0.04
IAC	200 copies	24.94	23.82		24.77		23.83		23.69

^aData were shown as average of two independent experiment
^bUD refers to "Underdetermined", a negative detection result
^cAbout 200 copies of pUC19 plasmid DNA was added as template for IAC to each multiplex real-time PCR reaction

Back to article page

ISSN: 1471-2180

Contact us

General enquiries: journalsubmissions@springernature.com