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Table 4 Detection results of the multiplex real-time PCR on the spiked food samples that inoculated with different concentations of E. coli O157:H7 and enriched with different incubation time

From: Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli

Target gene Food matrice Incubation time (h)
  CFU/gram 0 4   8   12   24  
  Beef   CT a ±SD CT ±SD CT ±SD CT ±SD
Z3276 80 UDb 34.50 0.29 28.43 0.07 27.98 0.10 27.98 0.10
800 UD 30.51 0.38 24.54 0.10 24.00 0.04 24.00 0.04
stx1 80 UD 27.54 0.19 21.73 0.22 20.84 0.25 19.06 0.36
800 UD 24.45 0.22 18.51 0.14 17.46 0.03 18.48 0.02
stx2 80 UD 31.34 0.10 25.47 0.02 25.03 0.04 23.63 0.02
800 UD 27.93 0.34 21.68 0.03 21.33 0.06 22.52 0.05
IAC 200 copiesc 28.88 27.72   21.96   21.09   19.59  
Spinach   CT ±SD CT ±SD CT ±SD CT ±SD
Z3276 80 UD 33.92 0.04 24.32 0.26 22.86 0.15 24.26 0.09
800 UD 30.91 0.05 23.04 0.11 23.08 0.08 23.12 0.07
stx1 80 UD 27.63 0.16 17.32 0.15 15.74 0.13 16.87 0.11
800 UD 25.04 0.06 16.57 0.12 16.25 0.15 16.37 0.10
stx2 80 UD 30.88 0.06 21.21 0.07 20.10 0.06 21.64 0.07
800 UD 28.19 0.14 20.05 0.15 20.58 0.05 20.74 0.04
IAC 200 copies 24.94 23.82   24.77   23.83   23.69  
  1. aData were shown as average of two independent experiment
  2. bUD refers to "Underdetermined", a negative detection result
  3. cAbout 200 copies of pUC19 plasmid DNA was added as template for IAC to each multiplex real-time PCR reaction