Table 3 Primers and probes used in the multiplex real-time PCR assay
Target gene | Primer/Probe | Sequence (5' -- 3') | Amplicon | Reference |
|---|---|---|---|---|
length (bp) | Â | |||
Z3276 | Z3276 forward | TATTCCGCGATGCTTGTTTTT | 130 | Li and Chen. 2012 |
Z3276 reverse | ATTATCTCACCAGCAAACTGGCGG | |||
Z3276 probe | FAM-CCCGCAAATCTTTCCMGBNFQ | |||
stx1 | stx1 forward | GGATTTCGTACAACACTGGATGAT | 67 | This study |
stx1 reverse | ATCCACATCTTCAGCAGTCATTACA | |||
stx1 probe | TAMRA-CAGTGGGCGTTCTTMGBNFQ | |||
stx2 | stx2 forward | GGGCAGTTATTTTGCTGTGGAT | 59 | This study |
stx2 reverse | GGTCAAAACGCGCCTGAT | |||
stx2 probe | JOE-ACGAGGGCTTGATGTCMGBNFQ | |||
IAC | IAC forward | CAGGATTAGCAGAGCGAGGTATG | 65 | Fricker et al. 2007 |
IAC reverse | CGTAGTTAGGCCACCACTTCAAG | |||
IAC probe | CY5-AGGCGGTGCTACAGAG-MGBNFQ |