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Table 2 Detection results of the exclusivity test with different bacterial strains by the multiplex real-time PCR assay

From: Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli

    

Target gene

Genus

Species

Pathotype

Strain name/Serotype

Z3276

stx1

stx2

Escherichia

coli

EHEC

EDL933/O157:H7

+

+

+

Escherichia

coli

ETEC

EC1775/O26:H11

_

+

_

Escherichia

coli

STEC

EC1803/O103:H11

_

+

+

Escherichia

coli

STEC

EC1807/O121:H19

_

_

+

Escherichia

coli

ETEC

EC1801

_

+

_

Escherichia

coli

EPEC

EC1501

_

_

_

Escherichia

coli

EIEC

EC1513

_

_

_

Escherichia

coli

EDC

DEC5A

_

_

_

Escherichia

coli

 

K12/MG1655

_

_

_

    

_

_

_

Salmonella

enterica

 

SL192/Typhi

_

_

_

Salmonella

enterica

 

SL317/Newport

_

_

_

Salmonella

enterica

 

SL535/Typhimurim

_

_

_

    

_

_

_

Shigella

sonnei

 

SH20145

_

_

_

Shigella

dysenteriae

 

SH20152

_

_

_

Shigella

flexneri

 

SH20155

_

_

_

Shigella

boydii

 

SH20140

_

_

_

    

_

_

_

Staphylococcus

aureus

 

ATCC25923

_

_

_

Staphylococcus

epidermidis

 

ATCC12228

_

_

_

Staphylococcus

pyogenes

 

ATCC19615

_

_

_

    

_

_

_

Vibrio

alginolytica

 

ATCC17749

_

_

_

Vibrio

parahemolyticus

 

ATCC17802

_

_

_

Vibrio

vulasfians

 

ATCC27562

_

_

_

    

_

_

_

Enterobacter

cloacae

 

ATTCC23355

_

_

_

Enterobacter

cloacae

 

ATCC13047

_

_

_

Enterobacter

cloacae

 

ATCC13048

_

_

_

    

_

_

_

Citrobacter

freundii

 

ATCC8090

_

_

_

    

_

_

_

Klebsiella

pneumoniae

 

ATCC13883

_

_

_

    

_

_

_

Pseudomonas

aeruginosa

 

ATCC27853

_

_

_