Fig. 1

a Determination of intracellular ROS accumulation of cells exposed to 1 mM H₂O₂. The histogram shows ROS accumulation detected as green fluorescence from ROS-dependent oxidation of the ROS-sensitive probe H₂DCF-DA following 30 min of incubation in the presence of H₂O₂. The experiment was repeated twice in triplicates; the histogram here shows fluorescence values from one representative replica. b Viability of cells following 2 and 8 h of exposure to 1 mM H₂O₂ measured as cells with intact membranes not stained by propidium iodide. Data are mean values from triplicate cultures from one representative experiment (the experiment was repeated twice). Error bars represents standard deviations