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Fig. 3 | BMC Microbiology

Fig. 3

From: Strain-dependent interactions of Streptococcus gallolyticus subsp. gallolyticus with human blood cells

Fig. 3

Bacterial titer and induction of gene expression of IL1B (a), IL6 (b) and IL8 (c). THP-1 cells were inoculated with 2.4–7.1 × 105 CFU/ml of S. gallolyticus subsp. gallolyticus strains. After 6 h of inoculation, the bacterial titer was determined by plating assay in triplicate (n = 3) and displayed as black dots in panel A. The bacterial titers are shown on the right-hand y-axis, and the horizontal grey line represents the input titer. LTA (25 μg/ml) and media were used as positive and negative controls, respectively (bars; n = 3). After 6 h of inoculation, the relative gene expression of IL1B, IL6 and IL8 was determined using real-time PCR. Comparison of induction between the isolates (lines above the bars) was performed using the Mann-Whitney test (n.s: not significant, *: p ≤ 0.05, **: p ≤ 0.005, ***: p ≤ 0.0005)

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