Fig. 5

Heatmap of significantly different metabolic pathways per time-point. KEGG metabolic pathways significantly correlating with AGS for microbial communities of Ca1 and Ca2. Pathways were determined by MEGAN classification. KEGG pathways abundances were normalised with DESEQ2 [96] and correlated to the AGS using the Spearman correlation method with the False Discovery Rate (FDR) test to calculate probabilities (FDR cut-off at 0.05). KEGG-pathways correlating positively or negatively with a p-value <0.05 are shown for Ca1 (blue) and Ca2 (red) for all the time-points, the AGS is shown in the top panel. # indicates pathways that are significant in both Ca1 and Ca2, § indicates pathways that are significant in Ca2, and the rest are only significant in Ca1. Pathway abundances were centred and scaled per row and positive and negative correlations were clustered based on the sign of the Spearman correlations