Fig. 1
From: Temporal dynamics in microbial soil communities at anthrax carcass sites
Estimation of B. anthracis abundance in soil samples using qPCR, metagenomic reads mapping and cultivation. For all panels, sampling time-points are on the x-axis. a and d qPCR results with estimated number of B. anthracis genomes per gram soil along the y-axis at different time-points (x-axis) for Carcass 1 (Ca1) (a) and Carcass 2 (Ca2) (d). The spiked sample is control soil from day 0 with 3.4 × 106 Stern34F2 B. anthracis spores added. P1 and P2 represent the two replicates at each time-point. b, c, e and f shows the results from mapping the metagenomic reads against the B. anthracis isolate genomes (K1 /K2) isolated from Ca1 and Ca2 as well as the other reference strains; B. cereus E33L, B. thuringiensis HD-771 and B. subtilis 168. Reads matching the references are on the y-axis. b and e and (c and f) shows the mapping of the metagenomes against the K1 and K2 strain, respectively as well as the other reference strains. Ca1 in panes (B and C) and Ca2 in panes (E and F). (G) B. anthracis spore counts of soil samples from Ca1 and Ca2 in spores per gram dry soil. Spore counts were estimated by culturing of heat-shocked soil samples and adjusted to dry weight of soil