Fig. 6

Colocalization of lysosomal proteins in M. abscessus CRM0019 phagosomes. a-f Z-stack images were obtained from A549 (a) and RAW (b) cells infected with M. abscessus CRM0019 for 24 h. a and b: Colocalization of mycobacteria-GFP, LAMP-1 and Cathepsin D, followed by the respective separated channels of CRM0019-GFP, Cathepsin D, LAMP-1 and transmitted light (DIC). c Quantification of M. abscessus CRM0019 and M. abscessus ATCC 19977 phagosomes simultaneously positive for LAMP-1 and cathepsin D in A549 and RAW cells. *P < 0.05. 200 phagosomes were counted per condition. Bar: 10 μm