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Table 4 Comparative summary of sequencing depth, reads length, and quality between GS FLEX, PGM, and MiSeq platforms

From: A comparison of sequencing platforms and bioinformatics pipelines for compositional analysis of the gut microbiome

Platform Raw reads Filtered readsa Mean Length after filtering (bp) Percentage of reads kept after filtering Mean quality score before quality filtering Number of identified OTUs
Roche 454 118,018 113,306 377 96% 37.7 1028
Ion Torrent PGM 481,593 71,652 297 14.9% 23.4 2747
Illumina Miseq 4,149,441 3,811,042 334 91.8% 37.5 3731
  1. aNumber of reads after filtering, reads of less than 200 nucleotides and with quality scores below 25 were removed