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Table 1 Absolute quantification of CpxAR, CpxP, and ArcBA by selected reaction monitoring

From: The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli

Peptide Protein Replicate Average amount [molecules/cell] SD [molecules/cell] CV [%]
AEDSPLGGLR CpxA wild type conditions 41* 11 28
  with 5 μg ml−1 gentamicin 32 6 19
nlpE overexpression (Cpx-ON) 78* 4 5
EHLSQEVLGK CpxR wild type conditions 393* 93 24
  with 5 μg ml−1 gentamicin 348 43 12
nlpE overexpression (Cpx-ON) 738* 69 9
DVTQWQK CpxP wild type conditions 36* 15 42
  with 5 μg ml−1 gentamicin 41 17 42
nlpE overexpression (Cpx-ON) 133* 28 21
FTQQGQVTVR ArcB wild type conditions 85 20 24
  with 5 μg ml−1 gentamicin 66 11 16
nlpE overexpression (Cpx-ON) 82 8 10
SLIGPDGEQYK ArcA wild type conditions 1088 162 15
  with 5 μg ml−1 gentamicin 796 150 19
nlpE overexpression (Cpx-ON) 484 41 8
  1. The peptides used for absolute determinations of protein molecules per cell are given as average values from five biological replicates with their standard deviations and coefficient of variance (CV) in percent for each of the four applied conditions
  2. Molecular amounts labeled with * represent data that were already published previously [32] but are mentioned here to provide complete information