Skip to main content

Table 1 Absolute quantification of CpxAR, CpxP, and ArcBA by selected reaction monitoring

From: The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli

Peptide

Protein

Replicate

Average amount [molecules/cell]

SD [molecules/cell]

CV [%]

AEDSPLGGLR

CpxA

wild type conditions

41*

11

28

 

with 5 μg ml−1 gentamicin

32

6

19

nlpE overexpression (Cpx-ON)

78*

4

5

EHLSQEVLGK

CpxR

wild type conditions

393*

93

24

 

with 5 μg ml−1 gentamicin

348

43

12

nlpE overexpression (Cpx-ON)

738*

69

9

DVTQWQK

CpxP

wild type conditions

36*

15

42

 

with 5 μg ml−1 gentamicin

41

17

42

nlpE overexpression (Cpx-ON)

133*

28

21

FTQQGQVTVR

ArcB

wild type conditions

85

20

24

 

with 5 μg ml−1 gentamicin

66

11

16

nlpE overexpression (Cpx-ON)

82

8

10

SLIGPDGEQYK

ArcA

wild type conditions

1088

162

15

 

with 5 μg ml−1 gentamicin

796

150

19

nlpE overexpression (Cpx-ON)

484

41

8

  1. The peptides used for absolute determinations of protein molecules per cell are given as average values from five biological replicates with their standard deviations and coefficient of variance (CV) in percent for each of the four applied conditions
  2. Molecular amounts labeled with * represent data that were already published previously [32] but are mentioned here to provide complete information