Samples | Pathogen | Method | No of positives/ total no of replicates (%) | Total | ||||
---|---|---|---|---|---|---|---|---|
101 CFU/25 g | 102 CFU/25 g | 103 CFU/25 g | 104 CFU/25 g | 105 CFU/25 g | ||||
Minced beef (n = 5) | S. Typhi | IC-culture | 2/15 (13.3) | 5/15 (33.3) | 9/15 (60) | 11/15 (73.3) | 15/15 (100) | 42/75 (56) a |
Culture | 0/15 (0) | 1/15 (6.7) | 5/15 (33.3) | 10/15 (66.7) | 14/15 (93.3) | 30/75 (40) | ||
IC-PCR | 3/15 (20) | 6/15 (40) | 10/15 (66.7) | 13/15 (86.7) | 15/15 (100) | 47/75 (62.7) | ||
PCR | 3/15 (20) | 5/15 (33.3) | 9/15 (60) | 11/15 (73.3) | 14/15 (93.3) | 42/75 (56) | ||
Minced chicken (n = 6) | S. flexneri | IC-culture | 3/18 (16.7) | 7/18 (38.9) | 11/18 (61.1) | 15/18 (83.3) | 18/18 (100) | 54/90 (60) a |
Culture | 0/18 (0) | 2/18 (11.1) | 6/18 (33.3) | 11/18 (61.1) | 16/18 (88.8) | 35/90 (38.8) | ||
IC-PCR | 3/18 (16.7) | 9/18 (50) | 14/18 (77.8) | 17/18 (94.4) | 18/18 (100) | 61/90 (67.8) | ||
PCR | 2/18 (11.1) | 6/18 (33.3) | 12/18 (66.7) | 15/18 (83.3) | 18/18 (100) | 53/90 (58.8) | ||
Minced fish (n = 5) | V. cholerae | IC-culture | 3/15 (20) | 6/15 (40) | 9/15 (60) | 12/15 (80) | 15/15 (100) | 45/75 (60) a |
Culture | 0/15 (0) | 2/15 (13.3) | 5/15 (33.3) | 11/15 (73.3) | 14/15 (93.3) | 32/75 (42.7) | ||
IC-PCR | 3/15 (20) | 7/15 (46.7) | 11/15 (73.3) | 13/15 (86.7) | 15/15 (100) | 49/75 (65.3) | ||
PCR | 3/15 (20) | 7/15 (46.7) | 10/15 (66.7) | 11/15 (73.3) | 13/15 (86.7) | 44/75 (58.7) | ||
Minced shrimpb (n = 5) | S. Typhi S. flexneri V. cholerae | IC-culture | 4/15 (26.7) | 7/15 (46.7) | 10/15 (66.7) | 13/15 (86.7) | 15/15 (100) | 49/75 (65.3) a |
Culture | 0/15 (0) | 2/15 (13.3) | 6/15 (40) | 9/15 (60) | 13/15 (86.7) | 30/75 (40) | ||
IC-PCR | 5/15 (33.3) | 9/15 (60) | 11/15 (73.3) | 12/15 (80) | 15/15 (100) | 52/75 (69.3) | ||
PCR | 3/15 (20) | 6/15 (40) | 9/15 (60) | 10/15 (66.7) | 11/15 (73.3) | 39/75 (52) | ||
MIC = Microplate immunocapture method aStatistically significantly different (p = 0.024) according to ANOVA bOnly the samples in which all three pathogen detected are included |