Fig. 5

IRAK-M affected Hif-1 and MAPK signaling cascade during M. tb infection. a Human samples from pulmonary tuberculosis (n = 6) were collected for Hif-1α detection by immunohistochemistry (400 ×, scale bar 50 μm). Para-carcinoma tissues of human lung cancer (n = 6), were used as negative control. Representative images were shown (400 ×, scale bar 10 μm). b U937 cells were infected with M. tb (MOI 10) at indicated time and expression of Hif-1α was evaluated by Western blot. c Lentivirus infected U937 cells (NC and KD) were infected with M. tb (MOI 10) for 24 h and Western blot was performed to detect Hif-1α, VEGF and phosphorylated ERK1/2. Densitometric analysis was performed using pooled data from three such experiments. Data were mean ± SD (**, p < 0.01; ***, p < 0.001)