Fig. 1

IRAK-M increased in M. tb infected macrophage cells and also in M. tb infected lung tissue. M. tb induced increased expression of IRAK-M in U937 cells. U937 cells were infected with M. tb H37Rv (MOI 10) for 24 h after seeding. a Cells grown on the coverslips were stained with anti-IRAK-M by immunocytochemistry. Representative images from three experiments were shown as merged results of IRAK-M (Cy3, red) and DAPI (blue) (1000×, scale bar 10 μm). b Expression of IRAK-M in U937 cells infected by inactivated or living M. tb H37Rv (MOI 10) was evaluated by Western blot. Densitometric analysis of IRAK-M expression was performed using pooled data from three such experiments. Results were expressed as Mean ± SD, ***: P < 0.001. c IRAK-M expression was increased in tissue of pulmonary tuberculosis. Human samples from pulmonary tuberculosis (n = 6) were collected for IRAK-M detection by immunohistochemistry (400×, scale bar 50 μm). Para-carcinoma tissues of human lung cancer (n = 6), were used as negative control. Representative images were shown