Table 1 Bacterial strains and plasmids used in this study
Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
Bacterial strains | ||
  Escherichia coli | ||
  BL21(DE3) | F¯ ompT hsdS(rB¯ mB¯) gal dcm λ (DE3) | Novagen |
  DH5α | F¯ ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK¯mK +) deoR thi-1 supE44 gyrA96 relA1 λ - phoA | Toyobo |
  Pseudomonas putida | ||
  KT2440 | Naturally Cmr | [34] |
  KT2440(pCAR1) | KT2440 harboring pCAR1 | [17] |
  KT2440(pCAR1pmrHis) | KT2440(pCAR1) carrying gene encoding His-tagged Pmr under original promotor | [13] |
Plasmids | ||
 pET-C-His-turA | pET-26b(+) with NdeI-XhoI fragment containing turA | [14] |
 pET-C-His-turB | pET-26b(+) with NdeI-SalI fragment containing turB ligated into the NdeI-XhoI site | [14] |
 pET-C-His-pmr | pET-26b(+) with NdeI-XhoI fragment containing pmr | [13] |
 pT7Blue T-vector | Apr, lacZα, T7 promoter, f1 origin, pUC/M13 priming sites | Novagen |
 pTuniv16S | pT7Blue T-vector with PCR fragment amplified from total DNA of Pseudomonas resinovorans CA10 with the primer set, univ16S-F and univ16S-R. | [19] |
 pTturA | pT7Blue T-vector with PCR fragment amplified from total DNA of KT2440 with the primer set, PP_1366-F and PP_1366-R. | This study |
 pTturB | pT7Blue T-vector with PCR fragment amplified from total DNA of KT2440 with the primer set, PP_3765-F and PP_3765-R. | This study |
 pTpmr2 | pT7Blue T-vector with PCR fragment amplified from pET-C-His-pmr with the primer set, pmr-F-2 and pmr-R-2. | This study |