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Table 1 Bacterial strains and plasmids used in this study

From: Growth phase-dependent expression profiles of three vital H-NS family proteins encoded on the chromosome of Pseudomonas putida KT2440 and on the pCAR1 plasmid

Strain or plasmid Relevant characteristics Source or reference
Bacterial strains
Escherichia coli
  BL21(DE3) ompT hsdS(rB¯ mB¯) gal dcm λ (DE3) Novagen
  DH5α F¯ ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK¯mK +) deoR thi-1 supE44 gyrA96 relA1 λ - phoA Toyobo
Pseudomonas putida
  KT2440 Naturally Cmr [34]
  KT2440(pCAR1) KT2440 harboring pCAR1 [17]
  KT2440(pCAR1pmrHis) KT2440(pCAR1) carrying gene encoding His-tagged Pmr under original promotor [13]
Plasmids
 pET-C-His-turA pET-26b(+) with NdeI-XhoI fragment containing turA [14]
 pET-C-His-turB pET-26b(+) with NdeI-SalI fragment containing turB ligated into the NdeI-XhoI site [14]
 pET-C-His-pmr pET-26b(+) with NdeI-XhoI fragment containing pmr [13]
 pT7Blue T-vector Apr, lacZα, T7 promoter, f1 origin, pUC/M13 priming sites Novagen
 pTuniv16S pT7Blue T-vector with PCR fragment amplified from total DNA of Pseudomonas resinovorans CA10 with the primer set, univ16S-F and univ16S-R. [19]
 pTturA pT7Blue T-vector with PCR fragment amplified from total DNA of KT2440 with the primer set, PP_1366-F and PP_1366-R. This study
 pTturB pT7Blue T-vector with PCR fragment amplified from total DNA of KT2440 with the primer set, PP_3765-F and PP_3765-R. This study
 pTpmr2 pT7Blue T-vector with PCR fragment amplified from pET-C-His-pmr with the primer set, pmr-F-2 and pmr-R-2. This study