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Fig. 3 | BMC Microbiology

Fig. 3

From: Growth phase-dependent expression profiles of three vital H-NS family proteins encoded on the chromosome of Pseudomonas putida KT2440 and on the pCAR1 plasmid

Fig. 3

Quantification of TurA and TurB monomers per cell in KT2440 and KT2440(pCAR1) cells. Panel a shows the growth curves of KT2440 and KT2440(pCAR1) in LB. The OD600 of the culture was measured every 2 h (circles) and cell number per milliliter was counted at the same time (triangles). Filled symbols represent the results of KT2440 and open symbols represent those of KT2440(pCAR1). In panels b and c, the numbers of TurA (b) and TurB (c) monomers per cell are shown. Values and error bars correspond to averages and standard deviations of results from at least three independent biological replicates. Gray bars represent the results of KT2440 and white bars represent those of KT2440(pCAR1). Note that the amount of TurB was below the detection limit at 2 h and 4 h in both KT2440 and KT2440(pCAR1). In panels d and e, corresponding mRNA levels of turA (d) and turB (e) genes are shown. Values and error bars correspond to averages and standard deviations of results from at least three independent technical replicates. Filled symbols and lines represent the results of KT2440 and open symbols and dot lines represent those of KT2440(pCAR1)

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BMC Microbiology

ISSN: 1471-2180

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