Table 1 Sequences of the primers used for phylogenetic and virulence potential analysis
Gene | Sequence (5′-3′) | Reference |
|---|---|---|
PCR | ||
16S rRNAF | AGAGTTTGATCATGGCTCAG | Borrell et al. [19] |
16S rRNAR | GGTTACCTTGTTACGACTT | Borrell et al. [19] |
gyrB 3F | TCCGGCGGTCTGCACGGCGT | Yáñez et al. [20] |
gyrB 14R | TTGTCCGGGTTGTACTCGTC | Yáñez et al. [20] |
hlyAF | GGCCGGTGGCCCGAAGATACGGG | Heuzenroeder et al. [21] |
hlyAR | GGCGGCGCCGGACGAGACGGG | Heuzenroeder et al. [21] |
altF | CCATCCCCAGCCTTTACGCCAT | Martínez et al. [22] |
altR | TTTCACCGAGGTGACGCCGT | Martínez et al. [22] |
astF | ATGCACGCACGTACCGCCAT | Martínez et al. [22] |
astR | ATCCGGTCGTCGCTCTTGGT | Martínez et al. [22] |
Sequencing | ||
16S BF | AGCAGTGGGGAATATTGCAC | This study |
16S BR | GGCAACAAAGGACAGGGGT | This study |
16S CF | ACGCAGGCGGTTGGATAAGT | This study |
16S CR | AAATCGACATCGTTTACGGCG | This study |
16S DF | AACCTTACCTGGCCTTGACA | This study |
16S DR | CACACACGCGGCATGGTGCATC | This study |
gyrB 9Rs | CCTTGACCGAAATGACCGCC | Yáñez et al. [20] |
gyrB 7F | GGGGTCTACTGCTTCACCAA | Yáñez et al. [20] |
gyrB 9R | ACCTTGACGGAGATAACGGC | Yáñez et al. [20] |